NAD+ + Pi  
 

      NADH + H+
Rxn#
Enzyme
DG°'
(kJ/mol)
DG
(kJ/mol)

1

2

3

4

5

6

7

8

9

10

Hexokinase

Phospho-glucoisomerase

Phosphofructo-kinase

Aldolase

Triose-P isomerase

Gliceraldehyde-3-P dehydrogenase Phosphoglycerate kinase

Phosphoglycerate mutase

Enolase

Pyruvate kinase

-16.7

+1.7

-14.2

+23.9

+7.6

+12.6

-37.6

+8.8

+3.4

-62.8

-33.5

-2.5

-22.2

-1.3

+2.5

-3.4

+2.6

+1.6

-6.6

-33.4

Free energy variation

(Mathews p.459).
Glucose + 2 ADP + 2 Pi + 2 NAD+  ---> 2 Pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O
Overview: 
Carbohydrate Metabolism
Glycolysis
Fructose-6-P (glucokinase) allosteric by the product(hexokinase)

regulation:

ATP e Acetyl-CoA

2-3,biPglicerate
reciprocal regulation of glycolysis and gluconeogenesis by pyruvate carboxylase
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