Enzyme - EC
1.2.1.12 Glyceraldehyde 3-phosphate dehydrogenase
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EC
1.2.1.12
Official
Name
glyceraldehyde-3-P
dehydrogenase
Alternative
name(s)
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
Triosephosphate dehydrogenase
GAPDH
Triosephosphate dehydrogenase
GAPDH
Class
1.Oxidoreductases
2.Acting on the aldehyde or oxo group of donors
1.With NAD+ or NADP+ as acceptor
2.Acting on the aldehyde or oxo group of donors
1.With NAD+ or NADP+ as acceptor
Reaction
catalysed
D-glyceraldehyde
3-P + P + NAD+ 
3-P-D-glycerol-P
+ NADH
3-P-D-glycerol-P
+ NADHSubstrates
D-glyceraldehyde 3-P
D-glyceraldehyde
orthophosphate
Tiol
NAD+
D-glyceraldehyde
orthophosphate
Tiol
NAD+
Products
3-P-D-glycerol-P
NADH
NADH
Metabolic Pathways
Other
comments
Also acts very slowly on D-glyceraldehyde and some other aldehydes. Thiols can replace phosphate.
Glyceraldehyde 3-phosphate dehydrogenase is a
tetrameric NAD-binding enzyme common to both the glycolytic and gluconeogenic
pathways. A cysteine in the middle of the molecule is involved in forming a
covalent phosphoglycerol thioester intermediate. The sequence around this
cysteine is totally conserved in eubacterial and eukaryotic GAPDHs and is
also present, albeit in a variant form, in the otherwise highly divergent
archaebacterial GAPDH.
Reference