HEADER NUCLEOTIDYLTRANSFERASE 10-JUN-96 1PEH TITLE NMR STRUCTURE OF THE MEMBRANE-BINDING DOMAIN OF CTP TITLE 2 PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE, 10 STRUCTURES COMPND MOL_ID: 1; COMPND 2 MOLECULE: PEPNH1; COMPND 3 CHAIN: NULL; COMPND 4 FRAGMENT: RESIDUES 236 - 268; COMPND 5 SYNONYM: CYTIDYLYLTRANSFERASE MEMBRANE BINDING DOMAIN COMPND 6 PEPTIDE; COMPND 7 EC: 2.7.7.15; COMPND 8 ENGINEERED: YES; COMPND 9 MUTATION: F263W SOURCE MOL_ID: 1; SOURCE 2 SYNTHETIC: YES; SOURCE 3 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS; SOURCE 4 ORGANISM_COMMON: RAT; SOURCE 5 ORGAN: LIVER KEYWDS TRANSFERASE, NUCLEOTIDYLTRANSFERASE, REPEAT, KEYWDS 2 PHOSPHOLIPID BIOSYNTHESIS, MEMBRANE, PHOSPHORYLATION EXPDTA NMR, 10 STRUCTURES AUTHOR S.J.DUNNE,R.B.CORNELL,J.E.JOHNSON,N.R.GLOVER,A.S.TRACEY REVDAT 1 07-DEC-96 1PEH 0 JRNL AUTH S.J.DUNNE,R.B.CORNELL,J.E.JOHNSON,N.R.GLOVER, JRNL AUTH 2 A.S.TRACEY JRNL TITL STRUCTURE OF THE MEMBRANE-BINDING DOMAIN OF CTP JRNL TITL 2 PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE JRNL REF BIOCHEMISTRY V. 35 1975 1996 JRNL REFN ASTM BICHAW US ISSN 0006-2960 0033 REMARK 1 REMARK 1 REFERENCE 1 REMARK 1 AUTH J.E.JOHNSON,R.B.CORNELL REMARK 1 TITL MEMBRANE-BINDING AMPHIPATHIC ALPHA-HELICAL PEPTIDE REMARK 1 TITL 2 DERIVED FROM CTP:PHOSPHOCHOLINE REMARK 1 TITL 3 CYTIDYLYLTRANSFERASE REMARK 1 REF BIOCHEMISTRY V. 33 4327 1994 REMARK 1 REFN ASTM BICHAW US ISSN 0006-2960 0033 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : DGII REMARK 3 AUTHORS : BIOSYM REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 1PEH COMPLIES WITH FORMAT V. 2.1, 25-OCT-1996 REMARK 210 REMARK 210 EXPERIMENTAL DETAILS REMARK 210 EXPERIMENT TYPE : NMR REMARK 210 TEMPERATURE (KELVIN) : 298 REMARK 210 PH : 5.20 REMARK 210 REMARK 210 NMR EXPERIMENTS CONDUCTED : COSY, TOCSY, NOESY REMARK 210 SPECTROMETER FIELD STRENGTH : 14.1 TESLA REMARK 210 SPECTROMETER MODEL : AMX600 REMARK 210 SPECTROMETER MANUFACTURER : BRUKER REMARK 210 REMARK 210 STRUCTURE DETERMINATION. REMARK 210 SOFTWARE USED : BIOSYM MOLECULAR REMARK 210 SIMULATIONS/FELIX REMARK 210 METHOD USED : RESTRAINED DISTANCE REMARK 210 GEOMETRY REMARK 210 REMARK 210 CONFORMERS, NUMBER CALCULATED : 10 REMARK 210 CONFORMERS, NUMBER SUBMITTED : 10 REMARK 210 CONFORMERS, SELECTION CRITERIA : ALL SUBMITTED REMARK 210 REMARK 210 REMARK: NULL REMARK 215 REMARK 215 NMR STUDY REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON REMARK 215 THESE RECORDS ARE MEANINGLESS. REMARK 999 REMARK 999 SEQUENCE REMARK 999 1PEH SWS P19836 1 - 234 NOT IN ATOMS LIST REMARK 999 1PEH SWS P19836 270 - 367 NOT IN ATOMS LIST REMARK 999 REMARK 999 PHE 263 WAS REPLACED WITH TRP FOR FLUORESCENCE STUDIES. DBREF 1PEH 235 269 SWS P19836 CTPT_RAT 235 269 SEQADV 1PEH ACE 235 SWS P19836 ILE 235 CONFLICT SEQADV 1PEH TRP 263 SWS P19836 PHE 263 ENGINEERED SEQADV 1PEH NH2 269 SWS P19836 GLU 269 CONFLICT SEQRES 1 35 ACE ASN GLU LYS LYS TYR HIS LEU GLN GLU ARG VAL ASP SEQRES 2 35 LYS VAL LYS LYS LYS VAL LYS ASP VAL GLU GLU LYS SER SEQRES 3 35 LYS GLU TRP VAL GLN LYS VAL GLU NH2 HET ACE 235 6 HET NH2 269 3 HETNAM ACE ACETYL GROUP HETNAM NH2 AMINO GROUP FORMUL 1 ACE C2 H3 O1 FORMUL 1 NH2 H2 N1 HELIX 1 1 LYS 248 LYS 252 5 5 HELIX 2 2 LYS 261 VAL 267 1 7 LINK C ACE 235 N ASN 236 LINK N NH2 269 C GLU 268 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000 MODEL 1 HETATM 1 C ACE 235 -26.069 -4.816 8.295 1.00 0.00 C HETATM 2 O ACE 235 -25.085 -5.431 8.701 1.00 0.00 O HETATM 3 CH3 ACE 235 -27.259 -4.555 9.228 1.00 0.00 C HETATM 4 1H ACE 235 -28.156 -5.017 8.817 1.00 0.00 H HETATM 5 2H ACE 235 -27.051 -4.982 10.211 1.00 0.00 H HETATM 6 3H ACE 235 -27.418 -3.481 9.328 1.00 0.00 H ATOM 7 N ASN 236 -26.110 -4.381 7.075 1.00 0.00 N ATOM 8 CA ASN 236 -25.052 -4.875 6.232 1.00 0.00 C ATOM 9 C ASN 236 -23.805 -4.012 6.246 1.00 0.00 C ATOM 10 O ASN 236 -22.898 -4.153 5.419 1.00 0.00 O ATOM 11 CB ASN 236 -25.599 -4.969 4.764 1.00 0.00 C ATOM 12 CG ASN 236 -25.190 -6.185 3.932 1.00 0.00 C ATOM 13 OD1 ASN 236 -25.376 -7.332 4.314 1.00 0.00 O ATOM 14 ND2 ASN 236 -24.622 -5.994 2.772 1.00 0.00 N ATOM 15 H ASN 236 -27.026 -4.167 6.734 1.00 0.00 H ATOM 16 HA ASN 236 -24.743 -5.873 6.585 1.00 0.00 H ATOM 17 1HB ASN 236 -26.705 -4.936 4.798 1.00 0.00 H ATOM 18 2HB ASN 236 -25.317 -4.049 4.221 1.00 0.00 H ATOM 19 1HD2 ASN 236 -24.399 -6.870 2.291 1.00 0.00 H ATOM 20 2HD2 ASN 236 -24.480 -5.029 2.475 1.00 0.00 H ATOM 21 N GLU 237 -23.756 -3.098 7.194 1.00 0.00 N ATOM 22 CA GLU 237 -22.627 -2.193 7.381 1.00 0.00 C ATOM 23 C GLU 237 -21.495 -2.882 8.106 1.00 0.00 C ATOM 24 O GLU 237 -20.304 -2.687 7.837 1.00 0.00 O ATOM 25 CB GLU 237 -23.144 -0.957 8.177 1.00 0.00 C ATOM 26 CG GLU 237 -22.180 -0.316 9.224 1.00 0.00 C ATOM 27 CD GLU 237 -21.596 1.070 8.946 1.00 0.00 C ATOM 28 OE1 GLU 237 -21.145 1.233 7.670 1.00 0.00 O ATOM 29 OE2 GLU 237 -21.538 1.951 9.794 1.00 0.00 O ATOM 30 H GLU 237 -24.628 -3.027 7.802 1.00 0.00 H ATOM 31 HA GLU 237 -22.263 -1.863 6.391 1.00 0.00 H ATOM 32 1HB GLU 237 -23.510 -0.206 7.453 1.00 0.00 H ATOM 33 2HB GLU 237 -24.075 -1.268 8.716 1.00 0.00 H ATOM 34 1HG GLU 237 -22.687 -0.254 10.209 1.00 0.00 H ATOM 35 2HG GLU 237 -21.320 -0.992 9.414 1.00 0.00 H ATOM 36 N LYS 238 -21.865 -3.705 9.072 1.00 0.00 N ATOM 37 CA LYS 238 -20.908 -4.433 9.922 1.00 0.00 C ATOM 38 C LYS 238 -19.818 -5.171 9.138 1.00 0.00 C ATOM 39 O LYS 238 -18.633 -5.231 9.469 1.00 0.00 O ATOM 40 CB LYS 238 -21.701 -5.445 10.796 1.00 0.00 C ATOM 41 CG LYS 238 -21.947 -4.949 12.240 1.00 0.00 C ATOM 42 CD LYS 238 -22.649 -5.946 13.164 1.00 0.00 C ATOM 43 CE LYS 238 -22.899 -7.256 12.403 1.00 0.00 C ATOM 44 NZ LYS 238 -24.346 -7.430 12.185 1.00 0.00 N ATOM 45 H LYS 238 -22.918 -3.761 9.242 1.00 0.00 H ATOM 46 HA LYS 238 -20.382 -3.696 10.512 1.00 0.00 H ATOM 47 1HB LYS 238 -22.693 -5.632 10.303 1.00 0.00 H ATOM 48 2HB LYS 238 -21.203 -6.431 10.774 1.00 0.00 H ATOM 49 1HG LYS 238 -20.993 -4.606 12.685 1.00 0.00 H ATOM 50 2HG LYS 238 -22.595 -4.044 12.218 1.00 0.00 H ATOM 51 1HD LYS 238 -22.034 -6.116 14.070 1.00 0.00 H ATOM 52 2HD LYS 238 -23.611 -5.519 13.517 1.00 0.00 H ATOM 53 1HE LYS 238 -22.359 -7.250 11.433 1.00 0.00 H ATOM 54 2HE LYS 238 -22.501 -8.127 12.964 1.00 0.00 H ATOM 55 1HZ LYS 238 -24.862 -7.108 13.016 1.00 0.00 H ATOM 56 2HZ LYS 238 -24.639 -6.879 11.365 1.00 0.00 H ATOM 57 3HZ LYS 238 -24.551 -8.426 12.019 1.00 0.00 H ATOM 58 N LYS 239 -20.275 -5.750 8.044 1.00 0.00 N ATOM 59 CA LYS 239 -19.454 -6.556 7.138 1.00 0.00 C ATOM 60 C LYS 239 -18.185 -5.807 6.680 1.00 0.00 C ATOM 61 O LYS 239 -17.062 -6.302 6.641 1.00 0.00 O ATOM 62 CB LYS 239 -20.303 -6.904 5.890 1.00 0.00 C ATOM 63 CG LYS 239 -20.333 -8.312 5.262 1.00 0.00 C ATOM 64 CD LYS 239 -19.189 -8.655 4.310 1.00 0.00 C ATOM 65 CE LYS 239 -18.594 -7.390 3.676 1.00 0.00 C ATOM 66 NZ LYS 239 -17.190 -7.652 3.297 1.00 0.00 N ATOM 67 H LYS 239 -21.295 -5.536 7.842 1.00 0.00 H ATOM 68 HA LYS 239 -19.082 -7.394 7.674 1.00 0.00 H ATOM 69 1HB LYS 239 -21.402 -6.615 6.073 1.00 0.00 H ATOM 70 2HB LYS 239 -20.046 -6.150 5.085 1.00 0.00 H ATOM 71 1HG LYS 239 -20.498 -9.103 6.019 1.00 0.00 H ATOM 72 2HG LYS 239 -21.280 -8.370 4.632 1.00 0.00 H ATOM 73 1HD LYS 239 -18.408 -9.237 4.856 1.00 0.00 H ATOM 74 2HD LYS 239 -19.559 -9.343 3.510 1.00 0.00 H ATOM 75 1HE LYS 239 -19.184 -7.085 2.788 1.00 0.00 H ATOM 76 2HE LYS 239 -18.634 -6.533 4.378 1.00 0.00 H ATOM 77 1HZ LYS 239 -16.932 -8.609 3.574 1.00 0.00 H ATOM 78 2HZ LYS 239 -17.088 -7.550 2.277 1.00 0.00 H ATOM 79 3HZ LYS 239 -16.578 -6.975 3.774 1.00 0.00 H ATOM 80 N TYR 240 -18.452 -4.591 6.320 1.00 0.00 N ATOM 81 CA TYR 240 -17.513 -3.682 5.664 1.00 0.00 C ATOM 82 C TYR 240 -16.632 -2.909 6.654 1.00 0.00 C ATOM 83 O TYR 240 -15.532 -3.322 7.018 1.00 0.00 O ATOM 84 CB TYR 240 -18.349 -2.777 4.678 1.00 0.00 C ATOM 85 CG TYR 240 -17.735 -2.584 3.287 1.00 0.00 C ATOM 86 CD1 TYR 240 -16.347 -2.504 3.141 1.00 0.00 C ATOM 87 CD2 TYR 240 -18.556 -2.501 2.153 1.00 0.00 C ATOM 88 CE1 TYR 240 -15.781 -2.351 1.877 1.00 0.00 C ATOM 89 CE2 TYR 240 -17.986 -2.350 0.889 1.00 0.00 C ATOM 90 CZ TYR 240 -16.602 -2.284 0.754 1.00 0.00 C ATOM 91 OH TYR 240 -16.043 -2.146 -0.485 1.00 0.00 O ATOM 92 H TYR 240 -19.478 -4.292 6.447 1.00 0.00 H ATOM 93 HA TYR 240 -16.813 -4.250 5.103 1.00 0.00 H ATOM 94 1HB TYR 240 -19.350 -3.314 4.479 1.00 0.00 H ATOM 95 2HB TYR 240 -18.728 -1.826 5.062 1.00 0.00 H ATOM 96 HD1 TYR 240 -15.704 -2.570 4.006 1.00 0.00 H ATOM 97 HD2 TYR 240 -19.629 -2.571 2.253 1.00 0.00 H ATOM 98 HE1 TYR 240 -14.711 -2.297 1.765 1.00 0.00 H ATOM 99 HE2 TYR 240 -18.627 -2.298 0.022 1.00 0.00 H ATOM 100 HH TYR 240 -15.262 -2.704 -0.532 1.00 0.00 H ATOM 101 N HIS 241 -17.154 -1.805 7.108 1.00 0.00 N ATOM 102 CA HIS 241 -16.569 -0.885 8.078 1.00 0.00 C ATOM 103 C HIS 241 -15.046 -0.908 8.000 1.00 0.00 C ATOM 104 O HIS 241 -14.240 -0.955 8.933 1.00 0.00 O ATOM 105 CB HIS 241 -17.200 -1.309 9.420 1.00 0.00 C ATOM 106 CG HIS 241 -16.250 -1.794 10.469 1.00 0.00 C ATOM 107 ND1 HIS 241 -15.999 -1.209 11.706 1.00 0.00 N ATOM 108 CD2 HIS 241 -15.493 -2.953 10.289 1.00 0.00 C ATOM 109 CE1 HIS 241 -15.092 -2.078 12.191 1.00 0.00 C ATOM 110 NE2 HIS 241 -14.736 -3.139 11.417 1.00 0.00 N ATOM 111 H HIS 241 -18.164 -1.688 6.831 1.00 0.00 H ATOM 112 HA HIS 241 -16.837 0.143 7.748 1.00 0.00 H ATOM 113 1HB HIS 241 -17.901 -0.535 9.791 1.00 0.00 H ATOM 114 2HB HIS 241 -17.942 -2.203 9.283 1.00 0.00 H ATOM 115 HD1 HIS 241 -16.399 -0.357 12.126 1.00 0.00 H ATOM 116 HD2 HIS 241 -15.515 -3.571 9.389 1.00 0.00 H ATOM 117 HE1 HIS 241 -14.688 -1.880 13.177 1.00 0.00 H ATOM 118 HE2 HIS 241 -14.063 -3.893 11.616 1.00 0.00 H ATOM 119 N LEU 242 -14.595 -0.808 6.741 1.00 0.00 N ATOM 120 CA LEU 242 -13.189 -0.839 6.347 1.00 0.00 C ATOM 121 C LEU 242 -12.712 0.549 5.995 1.00 0.00 C ATOM 122 O LEU 242 -12.174 0.810 4.908 1.00 0.00 O ATOM 123 CB LEU 242 -12.986 -1.807 5.143 1.00 0.00 C ATOM 124 CG LEU 242 -12.351 -3.195 5.416 1.00 0.00 C ATOM 125 CD1 LEU 242 -11.660 -3.724 4.150 1.00 0.00 C ATOM 126 CD2 LEU 242 -11.340 -3.167 6.577 1.00 0.00 C ATOM 127 H LEU 242 -15.340 -0.621 6.004 1.00 0.00 H ATOM 128 HA LEU 242 -12.592 -1.181 7.209 1.00 0.00 H ATOM 129 1HB LEU 242 -13.967 -1.968 4.650 1.00 0.00 H ATOM 130 2HB LEU 242 -12.374 -1.289 4.378 1.00 0.00 H ATOM 131 HG LEU 242 -13.161 -3.906 5.679 1.00 0.00 H ATOM 132 1HD1 LEU 242 -12.366 -3.800 3.300 1.00 0.00 H ATOM 133 2HD1 LEU 242 -10.826 -3.075 3.823 1.00 0.00 H ATOM 134 3HD1 LEU 242 -11.245 -4.737 4.304 1.00 0.00 H ATOM 135 1HD2 LEU 242 -10.538 -2.425 6.418 1.00 0.00 H ATOM 136 2HD2 LEU 242 -11.838 -2.912 7.533 1.00 0.00 H ATOM 137 3HD2 LEU 242 -10.863 -4.151 6.735 1.00 0.00 H ATOM 138 N GLN 243 -12.973 1.491 6.876 1.00 0.00 N ATOM 139 CA GLN 243 -12.523 2.870 6.681 1.00 0.00 C ATOM 140 C GLN 243 -11.249 3.218 7.409 1.00 0.00 C ATOM 141 O GLN 243 -10.480 4.094 6.968 1.00 0.00 O ATOM 142 CB GLN 243 -13.698 3.776 7.155 1.00 0.00 C ATOM 143 CG GLN 243 -14.313 4.680 6.036 1.00 0.00 C ATOM 144 CD GLN 243 -14.830 6.082 6.356 1.00 0.00 C ATOM 145 OE1 GLN 243 -14.867 6.497 7.507 1.00 0.00 O ATOM 146 NE2 GLN 243 -15.267 6.858 5.397 1.00 0.00 N ATOM 147 H GLN 243 -13.666 1.284 7.654 1.00 0.00 H ATOM 148 HA GLN 243 -12.334 3.003 5.595 1.00 0.00 H ATOM 149 1HB GLN 243 -14.545 3.138 7.551 1.00 0.00 H ATOM 150 2HB GLN 243 -13.352 4.324 8.051 1.00 0.00 H ATOM 151 1HG GLN 243 -13.600 4.743 5.188 1.00 0.00 H ATOM 152 2HG GLN 243 -15.185 4.144 5.581 1.00 0.00 H ATOM 153 1HE2 GLN 243 -15.608 7.773 5.698 1.00 0.00 H ATOM 154 2HE2 GLN 243 -15.223 6.453 4.461 1.00 0.00 H ATOM 155 N GLU 244 -10.991 2.589 8.538 1.00 0.00 N ATOM 156 CA GLU 244 -9.794 2.868 9.329 1.00 0.00 C ATOM 157 C GLU 244 -8.839 1.697 9.301 1.00 0.00 C ATOM 158 O GLU 244 -8.140 1.389 10.273 1.00 0.00 O ATOM 159 CB GLU 244 -10.230 3.205 10.782 1.00 0.00 C ATOM 160 CG GLU 244 -10.501 4.709 11.118 1.00 0.00 C ATOM 161 CD GLU 244 -9.759 5.335 12.300 1.00 0.00 C ATOM 162 OE1 GLU 244 -10.174 4.875 13.513 1.00 0.00 O ATOM 163 OE2 GLU 244 -8.876 6.172 12.166 1.00 0.00 O ATOM 164 H GLU 244 -11.724 1.896 8.871 1.00 0.00 H ATOM 165 HA GLU 244 -9.280 3.740 8.885 1.00 0.00 H ATOM 166 1HB GLU 244 -11.141 2.624 11.024 1.00 0.00 H ATOM 167 2HB GLU 244 -9.466 2.813 11.482 1.00 0.00 H ATOM 168 1HG GLU 244 -10.275 5.334 10.233 1.00 0.00 H ATOM 169 2HG GLU 244 -11.581 4.871 11.296 1.00 0.00 H ATOM 170 N ARG 245 -8.791 1.028 8.162 1.00 0.00 N ATOM 171 CA ARG 245 -7.885 -0.111 7.956 1.00 0.00 C ATOM 172 C ARG 245 -7.503 -0.214 6.475 1.00 0.00 C ATOM 173 O ARG 245 -6.365 -0.420 6.052 1.00 0.00 O ATOM 174 CB ARG 245 -8.555 -1.404 8.487 1.00 0.00 C ATOM 175 CG ARG 245 -8.029 -2.723 7.862 1.00 0.00 C ATOM 176 CD ARG 245 -7.636 -3.760 8.923 1.00 0.00 C ATOM 177 NE ARG 245 -8.623 -4.869 8.881 1.00 0.00 N ATOM 178 CZ ARG 245 -8.559 -5.926 8.083 1.00 0.00 C ATOM 179 NH1 ARG 245 -7.612 -6.137 7.218 1.00 0.00 N ATOM 180 NH2 ARG 245 -9.501 -6.797 8.175 1.00 0.00 N ATOM 181 H ARG 245 -9.408 1.403 7.381 1.00 0.00 H ATOM 182 HA ARG 245 -6.969 0.095 8.475 1.00 0.00 H ATOM 183 1HB ARG 245 -8.426 -1.469 9.587 1.00 0.00 H ATOM 184 2HB ARG 245 -9.655 -1.336 8.343 1.00 0.00 H ATOM 185 1HG ARG 245 -8.789 -3.179 7.195 1.00 0.00 H ATOM 186 2HG ARG 245 -7.157 -2.515 7.209 1.00 0.00 H ATOM 187 1HD ARG 245 -6.614 -4.142 8.730 1.00 0.00 H ATOM 188 2HD ARG 245 -7.623 -3.293 9.930 1.00 0.00 H ATOM 189 HE ARG 245 -9.424 -4.813 9.527 1.00 0.00 H ATOM 190 1HH1 ARG 245 -6.907 -5.399 7.209 1.00 0.00 H ATOM 191 2HH1 ARG 245 -7.672 -6.987 6.661 1.00 0.00 H ATOM 192 1HH2 ARG 245 -10.193 -6.538 8.882 1.00 0.00 H ATOM 193 2HH2 ARG 245 -9.458 -7.611 7.566 1.00 0.00 H ATOM 194 N VAL 246 -8.536 -0.042 5.676 1.00 0.00 N ATOM 195 CA VAL 246 -8.456 0.005 4.219 1.00 0.00 C ATOM 196 C VAL 246 -8.044 1.370 3.691 1.00 0.00 C ATOM 197 O VAL 246 -8.089 1.643 2.484 1.00 0.00 O ATOM 198 CB VAL 246 -9.851 -0.461 3.623 1.00 0.00 C ATOM 199 CG1 VAL 246 -10.543 0.513 2.642 1.00 0.00 C ATOM 200 CG2 VAL 246 -9.861 -1.836 2.900 1.00 0.00 C ATOM 201 H VAL 246 -9.474 -0.049 6.181 1.00 0.00 H ATOM 202 HA VAL 246 -7.649 -0.680 3.903 1.00 0.00 H ATOM 203 HB VAL 246 -10.562 -0.573 4.499 1.00 0.00 H ATOM 204 1HG1 VAL 246 -10.539 1.553 3.024 1.00 0.00 H ATOM 205 2HG1 VAL 246 -10.031 0.548 1.662 1.00 0.00 H ATOM 206 3HG1 VAL 246 -11.599 0.243 2.453 1.00 0.00 H ATOM 207 1HG2 VAL 246 -9.256 -2.598 3.433 1.00 0.00 H ATOM 208 2HG2 VAL 246 -10.880 -2.253 2.792 1.00 0.00 H ATOM 209 3HG2 VAL 246 -9.418 -1.785 1.887 1.00 0.00 H ATOM 210 N ASP 247 -7.598 2.237 4.577 1.00 0.00 N ATOM 211 CA ASP 247 -7.107 3.568 4.229 1.00 0.00 C ATOM 212 C ASP 247 -5.598 3.616 4.348 1.00 0.00 C ATOM 213 O ASP 247 -4.861 4.069 3.467 1.00 0.00 O ATOM 214 CB ASP 247 -7.774 4.626 5.150 1.00 0.00 C ATOM 215 CG ASP 247 -8.295 5.914 4.505 1.00 0.00 C ATOM 216 OD1 ASP 247 -7.589 6.893 4.301 1.00 0.00 O ATOM 217 OD2 ASP 247 -9.619 5.846 4.170 1.00 0.00 O ATOM 218 H ASP 247 -7.656 1.941 5.597 1.00 0.00 H ATOM 219 HA ASP 247 -7.373 3.749 3.175 1.00 0.00 H ATOM 220 1HB ASP 247 -8.614 4.148 5.697 1.00 0.00 H ATOM 221 2HB ASP 247 -7.067 4.907 5.962 1.00 0.00 H ATOM 222 N LYS 248 -5.124 3.162 5.502 1.00 0.00 N ATOM 223 CA LYS 248 -3.697 3.088 5.796 1.00 0.00 C ATOM 224 C LYS 248 -2.964 2.205 4.812 1.00 0.00 C ATOM 225 O LYS 248 -1.803 2.462 4.454 1.00 0.00 O ATOM 226 CB LYS 248 -3.502 2.566 7.249 1.00 0.00 C ATOM 227 CG LYS 248 -3.191 3.687 8.272 1.00 0.00 C ATOM 228 CD LYS 248 -3.823 5.045 7.952 1.00 0.00 C ATOM 229 CE LYS 248 -4.821 5.417 9.056 1.00 0.00 C ATOM 230 NZ LYS 248 -4.131 5.428 10.358 1.00 0.00 N ATOM 231 H LYS 248 -5.873 2.910 6.229 1.00 0.00 H ATOM 232 HA LYS 248 -3.270 4.105 5.706 1.00 0.00 H ATOM 233 1HB LYS 248 -4.410 2.020 7.573 1.00 0.00 H ATOM 234 2HB LYS 248 -2.693 1.806 7.265 1.00 0.00 H ATOM 235 1HG LYS 248 -3.492 3.360 9.287 1.00 0.00 H ATOM 236 2HG LYS 248 -2.099 3.859 8.329 1.00 0.00 H ATOM 237 1HD LYS 248 -3.029 5.812 7.848 1.00 0.00 H ATOM 238 2HD LYS 248 -4.338 4.996 6.970 1.00 0.00 H ATOM 239 1HE LYS 248 -5.276 6.409 8.850 1.00 0.00 H ATOM 240 2HE LYS 248 -5.667 4.699 9.090 1.00 0.00 H ATOM 241 1HZ LYS 248 -3.140 5.671 10.219 1.00 0.00 H ATOM 242 2HZ LYS 248 -4.572 6.125 10.975 1.00 0.00 H ATOM 243 3HZ LYS 248 -4.198 4.496 10.792 1.00 0.00 H ATOM 244 N VAL 249 -3.607 1.145 4.364 1.00 0.00 N ATOM 245 CA VAL 249 -2.987 0.191 3.444 1.00 0.00 C ATOM 246 C VAL 249 -2.719 0.798 2.088 1.00 0.00 C ATOM 247 O VAL 249 -1.884 0.293 1.314 1.00 0.00 O ATOM 248 CB VAL 249 -3.911 -1.088 3.311 1.00 0.00 C ATOM 249 CG1 VAL 249 -4.313 -1.439 1.860 1.00 0.00 C ATOM 250 CG2 VAL 249 -3.354 -2.408 3.911 1.00 0.00 C ATOM 251 H VAL 249 -4.618 1.024 4.681 1.00 0.00 H ATOM 252 HA VAL 249 -2.007 -0.110 3.860 1.00 0.00 H ATOM 253 HB VAL 249 -4.857 -0.864 3.865 1.00 0.00 H ATOM 254 1HG1 VAL 249 -4.668 -0.551 1.304 1.00 0.00 H ATOM 255 2HG1 VAL 249 -3.459 -1.840 1.280 1.00 0.00 H ATOM 256 3HG1 VAL 249 -5.121 -2.193 1.818 1.00 0.00 H ATOM 257 1HG2 VAL 249 -2.888 -2.258 4.904 1.00 0.00 H ATOM 258 2HG2 VAL 249 -4.136 -3.181 4.034 1.00 0.00 H ATOM 259 3HG2 VAL 249 -2.553 -2.849 3.287 1.00 0.00 H ATOM 260 N LYS 250 -3.411 1.868 1.746 1.00 0.00 N ATOM 261 CA LYS 250 -3.202 2.552 0.471 1.00 0.00 C ATOM 262 C LYS 250 -1.853 3.229 0.430 1.00 0.00 C ATOM 263 O LYS 250 -1.221 3.357 -0.630 1.00 0.00 O ATOM 264 CB LYS 250 -4.348 3.578 0.231 1.00 0.00 C ATOM 265 CG LYS 250 -4.278 4.262 -1.156 1.00 0.00 C ATOM 266 CD LYS 250 -5.008 3.520 -2.280 1.00 0.00 C ATOM 267 CE LYS 250 -6.512 3.493 -1.975 1.00 0.00 C ATOM 268 NZ LYS 250 -6.872 2.186 -1.400 1.00 0.00 N ATOM 269 H LYS 250 -4.167 2.179 2.425 1.00 0.00 H ATOM 270 HA LYS 250 -3.224 1.801 -0.338 1.00 0.00 H ATOM 271 1HB LYS 250 -5.328 3.077 0.345 1.00 0.00 H ATOM 272 2HB LYS 250 -4.315 4.340 1.035 1.00 0.00 H ATOM 273 1HG LYS 250 -4.728 5.272 -1.108 1.00 0.00 H ATOM 274 2HG LYS 250 -3.219 4.424 -1.437 1.00 0.00 H ATOM 275 1HD LYS 250 -4.801 4.012 -3.250 1.00 0.00 H ATOM 276 2HD LYS 250 -4.616 2.486 -2.363 1.00 0.00 H ATOM 277 1HE LYS 250 -6.781 4.313 -1.276 1.00 0.00 H ATOM 278 2HE LYS 250 -7.109 3.673 -2.893 1.00 0.00 H ATOM 279 1HZ LYS 250 -6.036 1.752 -0.981 1.00 0.00 H ATOM 280 2HZ LYS 250 -7.591 2.314 -0.673 1.00 0.00 H ATOM 281 3HZ LYS 250 -7.241 1.573 -2.141 1.00 0.00 H ATOM 282 N LYS 251 -1.380 3.676 1.579 1.00 0.00 N ATOM 283 CA LYS 251 -0.068 4.315 1.673 1.00 0.00 C ATOM 284 C LYS 251 1.022 3.324 2.012 1.00 0.00 C ATOM 285 O LYS 251 2.085 3.687 2.539 1.00 0.00 O ATOM 286 CB LYS 251 -0.130 5.450 2.733 1.00 0.00 C ATOM 287 CG LYS 251 0.081 6.864 2.137 1.00 0.00 C ATOM 288 CD LYS 251 1.502 7.416 2.275 1.00 0.00 C ATOM 289 CE LYS 251 1.562 8.820 1.656 1.00 0.00 C ATOM 290 NZ LYS 251 1.161 9.817 2.664 1.00 0.00 N ATOM 291 H LYS 251 -2.012 3.559 2.426 1.00 0.00 H ATOM 292 HA LYS 251 0.179 4.740 0.682 1.00 0.00 H ATOM 293 1HB LYS 251 -1.106 5.419 3.256 1.00 0.00 H ATOM 294 2HB LYS 251 0.623 5.260 3.526 1.00 0.00 H ATOM 295 1HG LYS 251 -0.148 6.860 1.054 1.00 0.00 H ATOM 296 2HG LYS 251 -0.645 7.569 2.589 1.00 0.00 H ATOM 297 1HD LYS 251 1.795 7.432 3.343 1.00 0.00 H ATOM 298 2HD LYS 251 2.219 6.742 1.764 1.00 0.00 H ATOM 299 1HE LYS 251 2.584 9.036 1.283 1.00 0.00 H ATOM 300 2HE LYS 251 0.895 8.896 0.773 1.00 0.00 H ATOM 301 1HZ LYS 251 1.465 9.505 3.597 1.00 0.00 H ATOM 302 2HZ LYS 251 1.602 10.722 2.446 1.00 0.00 H ATOM 303 3HZ LYS 251 0.137 9.924 2.655 1.00 0.00 H ATOM 304 N LYS 252 0.792 2.061 1.716 1.00 0.00 N ATOM 305 CA LYS 252 1.775 1.000 1.945 1.00 0.00 C ATOM 306 C LYS 252 2.272 0.401 0.651 1.00 0.00 C ATOM 307 O LYS 252 3.276 -0.331 0.623 1.00 0.00 O ATOM 308 CB LYS 252 1.129 -0.086 2.851 1.00 0.00 C ATOM 309 CG LYS 252 0.939 0.394 4.317 1.00 0.00 C ATOM 310 CD LYS 252 2.220 0.410 5.153 1.00 0.00 C ATOM 311 CE LYS 252 1.896 0.573 6.643 1.00 0.00 C ATOM 312 NZ LYS 252 2.648 -0.437 7.416 1.00 0.00 N ATOM 313 H LYS 252 -0.173 1.815 1.343 1.00 0.00 H ATOM 314 HA LYS 252 2.644 1.450 2.458 1.00 0.00 H ATOM 315 1HB LYS 252 0.150 -0.383 2.439 1.00 0.00 H ATOM 316 2HB LYS 252 1.746 -1.003 2.834 1.00 0.00 H ATOM 317 1HG LYS 252 0.548 1.432 4.319 1.00 0.00 H ATOM 318 2HG LYS 252 0.152 -0.216 4.801 1.00 0.00 H ATOM 319 1HD LYS 252 2.801 -0.522 4.965 1.00 0.00 H ATOM 320 2HD LYS 252 2.880 1.244 4.812 1.00 0.00 H ATOM 321 1HE LYS 252 2.154 1.594 6.989 1.00 0.00 H ATOM 322 2HE LYS 252 0.811 0.452 6.834 1.00 0.00 H ATOM 323 1HZ LYS 252 2.861 -1.242 6.810 1.00 0.00 H ATOM 324 2HZ LYS 252 3.527 -0.021 7.757 1.00 0.00 H ATOM 325 3HZ LYS 252 2.081 -0.748 8.217 1.00 0.00 H ATOM 326 N VAL 253 1.584 0.670 -0.437 1.00 0.00 N ATOM 327 CA VAL 253 1.989 0.202 -1.776 1.00 0.00 C ATOM 328 C VAL 253 2.992 1.139 -2.440 1.00 0.00 C ATOM 329 O VAL 253 3.806 0.791 -3.298 1.00 0.00 O ATOM 330 CB VAL 253 0.703 0.070 -2.680 1.00 0.00 C ATOM 331 CG1 VAL 253 0.861 0.662 -4.101 1.00 0.00 C ATOM 332 CG2 VAL 253 0.096 -1.341 -2.889 1.00 0.00 C ATOM 333 H VAL 253 0.705 1.268 -0.347 1.00 0.00 H ATOM 334 HA VAL 253 2.524 -0.742 -1.638 1.00 0.00 H ATOM 335 HB VAL 253 -0.092 0.690 -2.168 1.00 0.00 H ATOM 336 1HG1 VAL 253 1.350 1.653 -4.084 1.00 0.00 H ATOM 337 2HG1 VAL 253 1.492 0.020 -4.746 1.00 0.00 H ATOM 338 3HG1 VAL 253 -0.111 0.787 -4.614 1.00 0.00 H ATOM 339 1HG2 VAL 253 0.072 -1.935 -1.954 1.00 0.00 H ATOM 340 2HG2 VAL 253 -0.942 -1.305 -3.275 1.00 0.00 H ATOM 341 3HG2 VAL 253 0.686 -1.954 -3.597 1.00 0.00 H ATOM 342 N LYS 254 2.926 2.379 -1.996 1.00 0.00 N ATOM 343 CA LYS 254 3.806 3.459 -2.435 1.00 0.00 C ATOM 344 C LYS 254 5.202 3.272 -1.838 1.00 0.00 C ATOM 345 O LYS 254 6.262 3.476 -2.433 1.00 0.00 O ATOM 346 CB LYS 254 3.205 4.816 -1.981 1.00 0.00 C ATOM 347 CG LYS 254 3.164 5.905 -3.075 1.00 0.00 C ATOM 348 CD LYS 254 3.991 7.160 -2.774 1.00 0.00 C ATOM 349 CE LYS 254 3.668 8.238 -3.818 1.00 0.00 C ATOM 350 NZ LYS 254 4.712 9.278 -3.789 1.00 0.00 N ATOM 351 H LYS 254 2.197 2.536 -1.232 1.00 0.00 H ATOM 352 HA LYS 254 3.925 3.394 -3.504 1.00 0.00 H ATOM 353 1HB LYS 254 2.176 4.641 -1.576 1.00 0.00 H ATOM 354 2HB LYS 254 3.768 5.162 -1.078 1.00 0.00 H ATOM 355 1HG LYS 254 3.550 5.504 -4.033 1.00 0.00 H ATOM 356 2HG LYS 254 2.111 6.190 -3.280 1.00 0.00 H ATOM 357 1HD LYS 254 3.776 7.513 -1.747 1.00 0.00 H ATOM 358 2HD LYS 254 5.072 6.912 -2.800 1.00 0.00 H ATOM 359 1HE LYS 254 3.597 7.788 -4.831 1.00 0.00 H ATOM 360 2HE LYS 254 2.678 8.700 -3.622 1.00 0.00 H ATOM 361 1HZ LYS 254 5.586 8.878 -3.419 1.00 0.00 H ATOM 362 2HZ LYS 254 4.873 9.628 -4.745 1.00 0.00 H ATOM 363 3HZ LYS 254 4.407 10.055 -3.186 1.00 0.00 H ATOM 364 N ASP 255 5.164 2.847 -0.588 1.00 0.00 N ATOM 365 CA ASP 255 6.340 2.527 0.210 1.00 0.00 C ATOM 366 C ASP 255 7.052 1.323 -0.369 1.00 0.00 C ATOM 367 O ASP 255 8.268 1.284 -0.583 1.00 0.00 O ATOM 368 CB ASP 255 5.918 2.271 1.683 1.00 0.00 C ATOM 369 CG ASP 255 6.791 2.854 2.796 1.00 0.00 C ATOM 370 OD1 ASP 255 7.862 3.411 2.587 1.00 0.00 O ATOM 371 OD2 ASP 255 6.242 2.704 4.040 1.00 0.00 O ATOM 372 H ASP 255 4.167 2.704 -0.212 1.00 0.00 H ATOM 373 HA ASP 255 7.042 3.375 0.133 1.00 0.00 H ATOM 374 1HB ASP 255 4.869 2.591 1.849 1.00 0.00 H ATOM 375 2HB ASP 255 5.864 1.162 1.828 1.00 0.00 H ATOM 376 N VAL 256 6.253 0.293 -0.619 1.00 0.00 N ATOM 377 CA VAL 256 6.734 -0.935 -1.251 1.00 0.00 C ATOM 378 C VAL 256 6.922 -0.804 -2.752 1.00 0.00 C ATOM 379 O VAL 256 7.185 -1.786 -3.457 1.00 0.00 O ATOM 380 CB VAL 256 5.739 -2.116 -0.900 1.00 0.00 C ATOM 381 CG1 VAL 256 5.090 -2.892 -2.080 1.00 0.00 C ATOM 382 CG2 VAL 256 6.353 -3.209 0.007 1.00 0.00 C ATOM 383 H VAL 256 5.257 0.380 -0.252 1.00 0.00 H ATOM 384 HA VAL 256 7.746 -1.140 -0.851 1.00 0.00 H ATOM 385 HB VAL 256 4.879 -1.669 -0.326 1.00 0.00 H ATOM 386 1HG1 VAL 256 5.838 -3.383 -2.730 1.00 0.00 H ATOM 387 2HG1 VAL 256 4.389 -3.676 -1.736 1.00 0.00 H ATOM 388 3HG1 VAL 256 4.479 -2.234 -2.732 1.00 0.00 H ATOM 389 1HG2 VAL 256 6.795 -2.777 0.927 1.00 0.00 H ATOM 390 2HG2 VAL 256 5.595 -3.938 0.349 1.00 0.00 H ATOM 391 3HG2 VAL 256 7.154 -3.777 -0.500 1.00 0.00 H ATOM 392 N GLU 257 6.821 0.408 -3.258 1.00 0.00 N ATOM 393 CA GLU 257 7.083 0.714 -4.663 1.00 0.00 C ATOM 394 C GLU 257 8.159 1.773 -4.804 1.00 0.00 C ATOM 395 O GLU 257 8.340 2.387 -5.862 1.00 0.00 O ATOM 396 CB GLU 257 5.750 1.162 -5.323 1.00 0.00 C ATOM 397 CG GLU 257 4.845 0.045 -5.941 1.00 0.00 C ATOM 398 CD GLU 257 3.989 0.392 -7.161 1.00 0.00 C ATOM 399 OE1 GLU 257 4.390 0.277 -8.311 1.00 0.00 O ATOM 400 OE2 GLU 257 2.741 0.839 -6.846 1.00 0.00 O ATOM 401 H GLU 257 6.588 1.183 -2.573 1.00 0.00 H ATOM 402 HA GLU 257 7.470 -0.203 -5.141 1.00 0.00 H ATOM 403 1HB GLU 257 5.153 1.720 -4.576 1.00 0.00 H ATOM 404 2HB GLU 257 5.976 1.914 -6.104 1.00 0.00 H ATOM 405 1HG GLU 257 5.469 -0.823 -6.227 1.00 0.00 H ATOM 406 2HG GLU 257 4.158 -0.358 -5.172 1.00 0.00 H ATOM 407 N GLU 258 8.897 1.995 -3.735 1.00 0.00 N ATOM 408 CA GLU 258 10.029 2.923 -3.712 1.00 0.00 C ATOM 409 C GLU 258 11.328 2.154 -3.635 1.00 0.00 C ATOM 410 O GLU 258 12.379 2.576 -4.131 1.00 0.00 O ATOM 411 CB GLU 258 9.864 3.878 -2.498 1.00 0.00 C ATOM 412 CG GLU 258 9.138 5.241 -2.744 1.00 0.00 C ATOM 413 CD GLU 258 9.930 6.535 -2.542 1.00 0.00 C ATOM 414 OE1 GLU 258 11.146 6.597 -2.661 1.00 0.00 O ATOM 415 OE2 GLU 258 9.155 7.608 -2.219 1.00 0.00 O ATOM 416 H GLU 258 8.608 1.488 -2.846 1.00 0.00 H ATOM 417 HA GLU 258 10.028 3.500 -4.653 1.00 0.00 H ATOM 418 1HB GLU 258 9.328 3.338 -1.691 1.00 0.00 H ATOM 419 2HB GLU 258 10.866 4.078 -2.066 1.00 0.00 H ATOM 420 1HG GLU 258 8.740 5.270 -3.775 1.00 0.00 H ATOM 421 2HG GLU 258 8.238 5.315 -2.104 1.00 0.00 H ATOM 422 N LYS 259 11.260 0.988 -3.010 1.00 0.00 N ATOM 423 CA LYS 259 12.415 0.091 -2.927 1.00 0.00 C ATOM 424 C LYS 259 12.561 -0.800 -4.146 1.00 0.00 C ATOM 425 O LYS 259 13.279 -1.806 -4.135 1.00 0.00 O ATOM 426 CB LYS 259 12.294 -0.767 -1.637 1.00 0.00 C ATOM 427 CG LYS 259 13.609 -0.855 -0.824 1.00 0.00 C ATOM 428 CD LYS 259 13.573 -1.833 0.354 1.00 0.00 C ATOM 429 CE LYS 259 12.791 -1.200 1.513 1.00 0.00 C ATOM 430 NZ LYS 259 13.457 -1.523 2.787 1.00 0.00 N ATOM 431 H LYS 259 10.345 0.766 -2.517 1.00 0.00 H ATOM 432 HA LYS 259 13.322 0.722 -2.894 1.00 0.00 H ATOM 433 1HB LYS 259 11.494 -0.354 -0.989 1.00 0.00 H ATOM 434 2HB LYS 259 11.945 -1.788 -1.901 1.00 0.00 H ATOM 435 1HG LYS 259 14.441 -1.183 -1.476 1.00 0.00 H ATOM 436 2HG LYS 259 13.894 0.155 -0.468 1.00 0.00 H ATOM 437 1HD LYS 259 13.117 -2.789 0.033 1.00 0.00 H ATOM 438 2HD LYS 259 14.607 -2.072 0.674 1.00 0.00 H ATOM 439 1HE LYS 259 12.723 -0.099 1.380 1.00 0.00 H ATOM 440 2HE LYS 259 11.744 -1.566 1.537 1.00 0.00 H ATOM 441 1HZ LYS 259 14.478 -1.449 2.670 1.00 0.00 H ATOM 442 2HZ LYS 259 13.147 -0.863 3.514 1.00 0.00 H ATOM 443 3HZ LYS 259 13.213 -2.483 3.068 1.00 0.00 H ATOM 444 N SER 260 11.871 -0.451 -5.214 1.00 0.00 N ATOM 445 CA SER 260 11.957 -1.144 -6.498 1.00 0.00 C ATOM 446 C SER 260 12.608 -0.256 -7.535 1.00 0.00 C ATOM 447 O SER 260 13.168 -0.725 -8.534 1.00 0.00 O ATOM 448 CB SER 260 10.566 -1.640 -6.963 1.00 0.00 C ATOM 449 OG SER 260 10.609 -2.940 -7.563 1.00 0.00 O ATOM 450 H SER 260 11.190 0.354 -5.083 1.00 0.00 H ATOM 451 HA SER 260 12.614 -2.021 -6.360 1.00 0.00 H ATOM 452 1HB SER 260 9.874 -1.677 -6.100 1.00 0.00 H ATOM 453 2HB SER 260 10.101 -0.923 -7.669 1.00 0.00 H ATOM 454 HG SER 260 9.808 -3.403 -7.302 1.00 0.00 H ATOM 455 N LYS 261 12.522 1.047 -7.334 1.00 0.00 N ATOM 456 CA LYS 261 13.164 2.023 -8.215 1.00 0.00 C ATOM 457 C LYS 261 14.668 1.924 -8.107 1.00 0.00 C ATOM 458 O LYS 261 15.411 2.041 -9.088 1.00 0.00 O ATOM 459 CB LYS 261 12.669 3.453 -7.857 1.00 0.00 C ATOM 460 CG LYS 261 11.389 3.875 -8.621 1.00 0.00 C ATOM 461 CD LYS 261 11.551 4.011 -10.137 1.00 0.00 C ATOM 462 CE LYS 261 12.797 4.853 -10.440 1.00 0.00 C ATOM 463 NZ LYS 261 13.060 4.833 -11.890 1.00 0.00 N ATOM 464 H LYS 261 11.908 1.358 -6.522 1.00 0.00 H ATOM 465 HA LYS 261 12.887 1.788 -9.259 1.00 0.00 H ATOM 466 1HB LYS 261 12.480 3.519 -6.767 1.00 0.00 H ATOM 467 2HB LYS 261 13.481 4.185 -8.049 1.00 0.00 H ATOM 468 1HG LYS 261 10.569 3.164 -8.390 1.00 0.00 H ATOM 469 2HG LYS 261 11.034 4.858 -8.256 1.00 0.00 H ATOM 470 1HD LYS 261 11.619 3.006 -10.598 1.00 0.00 H ATOM 471 2HD LYS 261 10.649 4.492 -10.569 1.00 0.00 H ATOM 472 1HE LYS 261 12.656 5.897 -10.087 1.00 0.00 H ATOM 473 2HE LYS 261 13.684 4.468 -9.896 1.00 0.00 H ATOM 474 1HZ LYS 261 12.191 4.603 -12.394 1.00 0.00 H ATOM 475 2HZ LYS 261 13.397 5.758 -12.192 1.00 0.00 H ATOM 476 3HZ LYS 261 13.775 4.122 -12.099 1.00 0.00 H ATOM 477 N GLU 262 15.140 1.689 -6.894 1.00 0.00 N ATOM 478 CA GLU 262 16.564 1.528 -6.616 1.00 0.00 C ATOM 479 C GLU 262 17.141 0.282 -7.250 1.00 0.00 C ATOM 480 O GLU 262 18.372 0.097 -7.280 1.00 0.00 O ATOM 481 CB GLU 262 16.758 1.499 -5.070 1.00 0.00 C ATOM 482 CG GLU 262 18.081 2.107 -4.498 1.00 0.00 C ATOM 483 CD GLU 262 18.713 1.442 -3.272 1.00 0.00 C ATOM 484 OE1 GLU 262 17.804 0.978 -2.371 1.00 0.00 O ATOM 485 OE2 GLU 262 19.923 1.337 -3.120 1.00 0.00 O ATOM 486 H GLU 262 14.403 1.585 -6.128 1.00 0.00 H ATOM 487 HA GLU 262 17.108 2.392 -7.035 1.00 0.00 H ATOM 488 1HB GLU 262 15.899 2.009 -4.594 1.00 0.00 H ATOM 489 2HB GLU 262 16.673 0.447 -4.735 1.00 0.00 H ATOM 490 1HG GLU 262 18.856 2.120 -5.287 1.00 0.00 H ATOM 491 2HG GLU 262 17.934 3.173 -4.243 1.00 0.00 H ATOM 492 N TRP 263 16.303 -0.587 -7.776 1.00 0.00 N ATOM 493 CA TRP 263 16.760 -1.841 -8.372 1.00 0.00 C ATOM 494 C TRP 263 17.126 -1.680 -9.826 1.00 0.00 C ATOM 495 O TRP 263 18.007 -2.371 -10.359 1.00 0.00 O ATOM 496 CB TRP 263 15.617 -2.895 -8.228 1.00 0.00 C ATOM 497 CG TRP 263 15.928 -4.244 -8.876 1.00 0.00 C ATOM 498 CD1 TRP 263 16.634 -5.295 -8.221 1.00 0.00 C ATOM 499 CD2 TRP 263 15.644 -4.731 -10.140 1.00 0.00 C ATOM 500 NE1 TRP 263 16.772 -6.428 -9.046 1.00 0.00 N ATOM 501 CE2 TRP 263 16.150 -6.055 -10.227 1.00 0.00 C ATOM 502 CE3 TRP 263 14.999 -4.143 -11.258 1.00 0.00 C ATOM 503 CZ2 TRP 263 15.983 -6.809 -11.413 1.00 0.00 C ATOM 504 CZ3 TRP 263 14.854 -4.906 -12.429 1.00 0.00 C ATOM 505 CH2 TRP 263 15.332 -6.222 -12.499 1.00 0.00 C ATOM 506 H TRP 263 15.265 -0.363 -7.710 1.00 0.00 H ATOM 507 HA TRP 263 17.657 -2.192 -7.833 1.00 0.00 H ATOM 508 1HB TRP 263 15.403 -3.013 -7.115 1.00 0.00 H ATOM 509 2HB TRP 263 14.669 -2.448 -8.577 1.00 0.00 H ATOM 510 HD1 TRP 263 17.043 -5.235 -7.215 1.00 0.00 H ATOM 511 HE1 TRP 263 17.236 -7.321 -8.834 1.00 0.00 H ATOM 512 HE3 TRP 263 14.632 -3.125 -11.249 1.00 0.00 H ATOM 513 HZ2 TRP 263 16.352 -7.824 -11.472 1.00 0.00 H ATOM 514 HZ3 TRP 263 14.368 -4.475 -13.296 1.00 0.00 H ATOM 515 HH2 TRP 263 15.195 -6.791 -13.408 1.00 0.00 H ATOM 516 N VAL 264 16.463 -0.760 -10.493 1.00 0.00 N ATOM 517 CA VAL 264 16.699 -0.517 -11.928 1.00 0.00 C ATOM 518 C VAL 264 18.012 0.224 -12.172 1.00 0.00 C ATOM 519 O VAL 264 18.653 0.172 -13.224 1.00 0.00 O ATOM 520 CB VAL 264 15.491 0.304 -12.519 1.00 0.00 C ATOM 521 CG1 VAL 264 15.853 1.541 -13.386 1.00 0.00 C ATOM 522 CG2 VAL 264 14.485 -0.511 -13.360 1.00 0.00 C ATOM 523 H VAL 264 15.783 -0.146 -9.956 1.00 0.00 H ATOM 524 HA VAL 264 16.828 -1.481 -12.402 1.00 0.00 H ATOM 525 HB VAL 264 14.929 0.713 -11.630 1.00 0.00 H ATOM 526 1HG1 VAL 264 16.453 2.287 -12.833 1.00 0.00 H ATOM 527 2HG1 VAL 264 16.426 1.268 -14.293 1.00 0.00 H ATOM 528 3HG1 VAL 264 14.953 2.093 -13.723 1.00 0.00 H ATOM 529 1HG2 VAL 264 14.103 -1.391 -12.812 1.00 0.00 H ATOM 530 2HG2 VAL 264 13.594 0.090 -13.632 1.00 0.00 H ATOM 531 3HG2 VAL 264 14.930 -0.882 -14.304 1.00 0.00 H ATOM 532 N GLN 265 18.406 0.948 -11.152 1.00 0.00 N ATOM 533 CA GLN 265 19.600 1.738 -11.091 1.00 0.00 C ATOM 534 C GLN 265 20.846 0.958 -10.806 1.00 0.00 C ATOM 535 O GLN 265 21.971 1.389 -11.120 1.00 0.00 O ATOM 536 CB GLN 265 19.281 2.790 -9.934 1.00 0.00 C ATOM 537 CG GLN 265 17.939 3.563 -10.334 1.00 0.00 C ATOM 538 CD GLN 265 17.435 4.706 -9.466 1.00 0.00 C ATOM 539 OE1 GLN 265 16.304 5.155 -9.600 1.00 0.00 O ATOM 540 NE2 GLN 265 18.201 5.217 -8.531 1.00 0.00 N ATOM 541 H GLN 265 17.699 0.984 -10.354 1.00 0.00 H ATOM 542 HA GLN 265 19.724 2.309 -12.014 1.00 0.00 H ATOM 543 1HB GLN 265 18.936 2.093 -9.075 1.00 0.00 H ATOM 544 2HB GLN 265 19.965 3.243 -9.720 1.00 0.00 H ATOM 545 1HG GLN 265 18.065 3.870 -11.385 1.00 0.00 H ATOM 546 2HG GLN 265 17.150 2.793 -10.370 1.00 0.00 H ATOM 547 1HE2 GLN 265 17.777 5.967 -7.983 1.00 0.00 H ATOM 548 2HE2 GLN 265 19.127 4.792 -8.455 1.00 0.00 H ATOM 549 N LYS 266 20.701 -0.214 -10.218 1.00 0.00 N ATOM 550 CA LYS 266 21.852 -1.026 -9.814 1.00 0.00 C ATOM 551 C LYS 266 22.162 -2.137 -10.785 1.00 0.00 C ATOM 552 O LYS 266 23.333 -2.508 -10.989 1.00 0.00 O ATOM 553 CB LYS 266 21.577 -1.590 -8.389 1.00 0.00 C ATOM 554 CG LYS 266 21.349 -3.118 -8.302 1.00 0.00 C ATOM 555 CD LYS 266 21.488 -3.703 -6.889 1.00 0.00 C ATOM 556 CE LYS 266 20.301 -4.634 -6.609 1.00 0.00 C ATOM 557 NZ LYS 266 20.438 -5.209 -5.259 1.00 0.00 N ATOM 558 H LYS 266 19.709 -0.532 -10.006 1.00 0.00 H ATOM 559 HA LYS 266 22.735 -0.361 -9.783 1.00 0.00 H ATOM 560 1HB LYS 266 22.423 -1.312 -7.712 1.00 0.00 H ATOM 561 2HB LYS 266 20.700 -1.054 -7.943 1.00 0.00 H ATOM 562 1HG LYS 266 20.328 -3.375 -8.641 1.00 0.00 H ATOM 563 2HG LYS 266 22.033 -3.651 -8.992 1.00 0.00 H ATOM 564 1HD LYS 266 22.453 -4.238 -6.800 1.00 0.00 H ATOM 565 2HD LYS 266 21.515 -2.881 -6.146 1.00 0.00 H ATOM 566 1HE LYS 266 19.343 -4.080 -6.698 1.00 0.00 H ATOM 567 2HE LYS 266 20.247 -5.452 -7.357 1.00 0.00 H ATOM 568 1HZ LYS 266 21.031 -4.596 -4.682 1.00 0.00 H ATOM 569 2HZ LYS 266 19.508 -5.293 -4.825 1.00 0.00 H ATOM 570 3HZ LYS 266 20.871 -6.141 -5.325 1.00 0.00 H ATOM 571 N VAL 267 21.145 -2.702 -11.397 1.00 0.00 N ATOM 572 CA VAL 267 21.332 -3.846 -12.311 1.00 0.00 C ATOM 573 C VAL 267 21.829 -3.438 -13.699 1.00 0.00 C ATOM 574 O VAL 267 22.534 -4.150 -14.415 1.00 0.00 O ATOM 575 CB VAL 267 19.966 -4.623 -12.438 1.00 0.00 C ATOM 576 CG1 VAL 267 19.540 -4.937 -13.892 1.00 0.00 C ATOM 577 CG2 VAL 267 19.844 -5.973 -11.681 1.00 0.00 C ATOM 578 H VAL 267 20.177 -2.291 -11.252 1.00 0.00 H ATOM 579 HA VAL 267 22.126 -4.453 -11.904 1.00 0.00 H ATOM 580 HB VAL 267 19.174 -3.949 -12.012 1.00 0.00 H ATOM 581 1HG1 VAL 267 19.649 -4.054 -14.552 1.00 0.00 H ATOM 582 2HG1 VAL 267 20.164 -5.732 -14.343 1.00 0.00 H ATOM 583 3HG1 VAL 267 18.487 -5.265 -13.961 1.00 0.00 H ATOM 584 1HG2 VAL 267 20.267 -5.923 -10.657 1.00 0.00 H ATOM 585 2HG2 VAL 267 18.796 -6.312 -11.580 1.00 0.00 H ATOM 586 3HG2 VAL 267 20.405 -6.788 -12.176 1.00 0.00 H ATOM 587 N GLU 268 21.446 -2.238 -14.058 1.00 0.00 N ATOM 588 CA GLU 268 21.763 -1.594 -15.297 1.00 0.00 C ATOM 589 C GLU 268 23.080 -0.880 -15.243 1.00 0.00 C ATOM 590 O GLU 268 23.760 -0.649 -16.233 1.00 0.00 O ATOM 591 CB GLU 268 20.513 -0.613 -15.533 1.00 0.00 C ATOM 592 CG GLU 268 19.168 -1.485 -15.390 1.00 0.00 C ATOM 593 CD GLU 268 17.840 -0.824 -15.699 1.00 0.00 C ATOM 594 OE1 GLU 268 17.900 0.540 -15.786 1.00 0.00 O ATOM 595 OE2 GLU 268 16.796 -1.447 -15.862 1.00 0.00 O ATOM 596 H GLU 268 20.800 -1.769 -13.365 1.00 0.00 H ATOM 597 HA GLU 268 21.740 -2.305 -16.120 1.00 0.00 H ATOM 598 1HB GLU 268 20.510 0.003 -14.558 1.00 0.00 H ATOM 599 2HB GLU 268 20.617 -0.138 -16.197 1.00 0.00 H ATOM 600 1HG GLU 268 19.329 -2.370 -16.027 1.00 0.00 H ATOM 601 2HG GLU 268 19.199 -1.886 -14.363 1.00 0.00 H HETATM 602 N NH2 269 23.522 -0.483 -14.060 1.00 0.00 N HETATM 603 1HN NH2 269 22.969 -0.666 -13.208 1.00 0.00 H HETATM 604 2HN NH2 269 24.422 0.011 -13.986 1.00 0.00 H TER 605 NH2 269 ENDMDL END