Enzyme - EC
1.5.1.3 - Dihydrofolate reductase
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EC
1.5.1.3
Official
Name
Dihydrofolate reductase
Alternative
name(s)
Dihydrofolate reductase
Class
5.Acting on the CH-NH group of donors
1.With NAD+ or NADP+ as acceptor
Reaction
catalysed
5,6,7,8-tetrahydrofolate
+ NADP+ 
7,8-dihydrofolate
+ NADPH
7,8-dihydrofolate
+ NADPHSubstrates
5,6,7,8-tetrahydrofolate
folate
NADP+
folate
NADP+
Products
7,8-dihydrofolate
NADPH
NADPH
Inhibitors
Trimethoprim;
Methotrexate
Metabolic
Pathways
Other
comments
The enzyme from animals and some micro-organisms also slowly reduces folate to 5,6,7,8-tetrahydrofolate (EC 1.5.1.4).
Dihydrofolate reductases are ubiquitous enzymes which catalyze the reduction of folic acid into tetrahydrofolic acid. They can be inhibited by a number of antagonists such as trimethroprim and methotrexate which are used as antibacterial or anticancerous agents.
It has been derived a
signature pattern from a region in the N-terminal part of these enzymes, which
includes a conserved Pro-Trp dipeptide; the tryptophan has been shown to
be involved in the binding of substrate by the enzyme.
Reference